Site information for: LasR in species Pseudomonas aeruginosa; genome accession: NC

Transcription Factor Binding Site Information

dbxref: CollecTF:EXPSITE_00021950
Genome: Pseudomonas aeruginosa - NC_002516.2
TF: LasR [UniProtKB:P54292, view regulon]

Supporting Evidence

Binding site	Location	Publication	Experimental techniques used	Curation
CCTGTGAATTCCGGTAGTT	- [1224391, 1224409]	11359576	Experimental technique details Multiple sequence alignment (MSA) (ECO:0005556) Multiple sequence alignment (MSA) - ECO:0005556 MSA can be used to align promoter regions and highlight regions of conservation that may be indicative of transcription factor binding sites. - Experimental technique details RNAse protection (ECO:0000288) RNAse protection - ECO:0000288 This is a technique used to detect typically mRNA with greater precision than Northern blot or RT-PCR. Therefore, it is commonly used to assess gene expression (specifically transcription). RNAse protection uses labelled RNA probes to bind desired targets. RNAses are then added to the mix and degrade all RNA that is not bound to probes. The remaining RNA is typically run on a gel to detect the size (and label) of the probe and determine which RNA it is. -	734

Regulated genes

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Gene	Locus tag	Description
PA1131	PA1131	major facilitator superfamily (MFS) transporter
rhlC	PA1130	rhamnosyltransferase 2
PA1132	PA1132	hypothetical protein