Curation Information

The svpA-srtB locus of Listeria monocytogenes: fur-mediated iron regulation and effect on virulence.;Newton SM, Klebba PE, Raynaud C, Shao Y, Jiang X, Dubail I, Archer C, Frehel C, Charbit A;Molecular microbiology 2005 Feb; 55(3):927-40 [15661014]
Fur [Q8Y5U9, view regulon]
Reported TF sp.
Listeria monocytogenes EGD-e
Reported site sp.
Listeria monocytogenes EGD-e
Created by
Erill Lab
Curation notes

Experimental Process

The authors identify a putative Fur-box in the promoter region of lmo2186, and confirm with RT-PCR that this gene forms a single transcriptional unit with seven other genes (the svpA-srtB cluster, lmo2186-lmo2180). They verify using a Fur mutant and iron supplementation that Fur acts as a repressor of this operon via RT-PCR, Western immunoblotting and a GFP fusion. They further characterized the influence of this regulation in virulence phenotypes for L. monocytogenes.

Transcription Factor Binding Sites


Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
GACAATGATAATCATTATC lmo2180, lmo2181, lmo2182, lmo2183, lmo2184, lmo2185, lmo2186
... ... lmo2180 lmo2181 lmo2182 lmo2183 lmo2184 lmo2185 lmo2186
Experimental technique details Ad-hoc quantitative phenotype observation (ECO:0005676) - Experimental technique details GFP Promoter Fusion (ECO:0005636) - Experimental technique details qRT-PCR [RNA] (ECO:0001808) - Experimental technique details Visual sequence inspection (nan) - Experimental technique details Western blot (quantitative) expression analysis (ECO:0000279) - repressor not specified