The authors identify a putative Fur-box in the promoter region of lmo2186, and confirm with RT-PCR that this gene forms a single transcriptional unit with seven other genes (the svpA-srtB cluster, lmo2186-lmo2180). They verify using a Fur mutant and iron supplementation that Fur acts as a repressor of this operon via RT-PCR, Western immunoblotting and a GFP fusion. They further characterized the influence of this regulation in virulence phenotypes for L. monocytogenes.
Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.
|Site sequence||Regulated genes||Gene diagram||Experimental techniques||TF function||TF type|
|GACAATGATAATCATTATC||lmo2180, lmo2181, lmo2182, lmo2183, lmo2184, lmo2185, lmo2186||