Curation Information

Publication
Identification of additional genes belonging to the LexA regulon in Escherichia coli.;Fernández De Henestrosa AR, Ogi T, Aoyagi S, Chafin D, Hayes JJ, Ohmori H, Woodgate R;Molecular microbiology 2000 Mar; 35(6):1560-72 [10760155]
TF
LexA [P0A7C2, view regulon]
Reported TF sp.
Escherichia coli str. K-12 substr. MG1655
Reported site sp.
Escherichia coli str. K-12 substr. MG1655
Created by
Kathryn Cronise
Curation notes
-

Experimental Process

A computational search was performed using 20 known LexA sites and the derived consensus sequence. A Northern blot analysis was used to verify expression.

Transcription Factor Binding Sites


TACTGTATGATTATCCAGTT
CACTGTATAAATAAACAGCT
AACTGATTAAAAACCCAGCG
TACTGTTTATTTATACAGTA
AGCTGTATTTGTCTCCAGTA
TACTGATGATATATACAGGT
CACTGGATAGATAACCAGCA
AACTGGATAAAATTACAGGG
TACTGTATATAAAAACAGTA
TACTGTATGCTCATACAGTA
TACTGTATGATTATCCAGTT
CACTGTATAAATAAACAGCT
AACTGATTAAAAACCCAGCG
TACTGTTTATTTATACAGTA
AGCTGTATTTGTCTCCAGTA
TACTGATGATATATACAGGT
CACTGGATAGATAACCAGCA
AACTGGATAAAATTACAGGG
TACTGTATATAAAAACAGTA
TACTGTATGCTCATACAGTA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TACTGTATGATTATCCAGTT dinQ,
... ... dinQ arsR
Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
CACTGTATAAATAAACAGCT hokE,
... ... hokE ybdK sokE
Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
AACTGATTAAAAACCCAGCG ybfE,
... ... ybfE ybfF
Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
TACTGTTTATTTATACAGTA tisB,
... ... tisB istR
Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
AGCTGTATTTGTCTCCAGTA insK,
... ... insK insJ sokA
Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
TACTGATGATATATACAGGT symE,
... ... symE symR hsdS hsdM
Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
CACTGGATAGATAACCAGCA cho,
... ... cho nadE ves
Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
AACTGGATAAAATTACAGGG yehH,
... ... yehH metG yehI
Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
TACTGTATATAAAAACAGTA umuD, umuC,
... ... umuD umuC hlyE dsbB
Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
TACTGTATGCTCATACAGTA recA,
... ... recA ygaD
Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer