Curation Information

Publication
Identification of additional genes belonging to the LexA regulon in Escherichia coli.;Fernández De Henestrosa AR, Ogi T, Aoyagi S, Chafin D, Hayes JJ, Ohmori H, Woodgate R;Molecular microbiology 2000 Mar; 35(6):1560-72 [10760155]
TF
LexA [P0A7C2, view regulon]
Reported TF sp.
Escherichia coli str. K-12 substr. MG1655
Reported site sp.
Escherichia coli str. K-12 substr. MG1655
Created by
Kathryn Cronise
Curation notes
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Experimental Process

A computational search was performed using 20 known sites to obtain predicited sites. Northern blot analysis was performed to confirm expression. EMSAs and hydroxyl radical footprinting were performed to confirm binding.

Transcription Factor Binding Sites


TACTGTACGTATCGACAGTT
CACTGTATAAAAATCCTATA
TACTGTACGTATCGACAGTT
CACTGTATAAAAATCCTATA

Gene Regulation

Regulated genes for each binding site are displayed below. Gene regulation diagrams show binding sites, positively-regulated genes, negatively-regulated genes, both positively and negatively regulated genes, genes with unspecified type of regulation. For each indvidual site, experimental techniques used to determine the site are also given.

Site sequence Regulated genes Gene diagram Experimental techniques TF function TF type
TACTGTACGTATCGACAGTT ydjM,
... ... ydjM yniC
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Hydroxyl-radical footprinting (ECO:0005643) - Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer
CACTGTATAAAAATCCTATA ydjM,
... ... ydjM yniC
Experimental technique details EMSA (ECO:0001807) - Experimental technique details Hydroxyl-radical footprinting (ECO:0005643) - Experimental technique details Northern blot (ECO:0005653) - Experimental technique details PSSM site search (ECO:0005659) - repressor dimer