A quantifiable phenotypic trait (e.g. glucose intake) is assessed in a quantitative manner after induction of the regulatory mechanism and used as a natural reporter.. The observable phenotypic trait should be described in the experimental notes.

Target promoter is inserted in front of a GFP gene, and the target TF is induced. Expression levels of GFP should thereby depend on the target TF.

Quantitative Reverse-Transcription PCR is a modification of PCR in which RNA is first reverse transcribed into cDNA and this is amplified measuring the product (qPCR) in real time. It therefore allows one to analyze transcription by directly measuring the product (RNA) of a gene's transcription. If the gene is transcribed more, the starting product for PCR is larger and the corresponding volume of amplification is also larger.

Visual inspection of promoter region to identify putative binding sites based on previous knowledge

A Western blot (also frequently called immunoblot) is a gel-based technique used to determine the presence of specific proteins in a sample by means of separation (through gel electrophoresis) and detection (using a protein-specific antibody). Less frequent than the equivalent RNA Northern blot, it is typically used in TF-binding site analysis to validate differential regulation of target genes.